A Review on Telomerase Activity Detection Strategies

KeyWord:telomerase activity  chemiluminescence  electrochemical method  fluorescence analysis method  colorimetry

Abstract:

      Telomerase is a ribonucleoprotein which can catalyze the addition of single strand repeat units£¨TTAGGG£©n onto the 3'ends of telomeres in the eukaryotic chromosomes£¬preserving telomeric length and stability£®Compared to human somatic cells£¬it is testified that telomerase activity is overexpressed in over 85% of all known human cancer cells£¬indicating that telomerase would be used as a biomarker for the detection of tumor cells£®Therefore£¬a reliable method for telomerase detecting plays a crucial role in the diagnosis and treatment of malignant tumors£®Since telomerase was discovered in 1985£¬telomerase activity detection methods have made great breakthroughs in the following 30 years of continuous reform and development£®In this paper£¬the methods for detecting telomerase activity in the past three years were reviewed£¬such as chemiluminescence method£¬electrochemistry method£¬fluorescence analysis method as well as colorimetry£®To cut the long story short£¬the aim of this paper is to have a more comprehensive understanding of the current research progress of telomerase activity detection£¬which may be of great help to the design of new telomerase activity detection protocols and provide some brand new ideas for overcoming the challenges of telomerase activity detection methods in clinical trials in the future.