Research Progress on Analytical Methods for Deciphering Adenosine-to-inosine RNA Editing

KeyWord:adenosine-to-inosine RNA editing  ?RNA modification  ?detection method  ?mapping analysis

Abstract:

      Adenosine-to-inosine£¨Ade-to-Ino£©RNA editing is one of the most widespread post-transcriptional modifications in RNA£®The conversion of adenosine to inosine is through the hydrolytic deamination of the amino group at the C6 position of adenosine£¬which is catalyzed by the adenosine deaminases acting on RNA£¨ADARs£©£®Accumulated lines of evidence show that Ade-to-Ino RNA editing is involved in the regulation of gene expression and protein functions£®Aberrant Ade-to-Ino RNA editing is demonstrated to be correlated with many human diseases£®In-depth investigation of the biological functions of Ade-to-Ino RNA editing depends on the sensitive detection£¬accurate quantification£¬and precise mapping analysis£®This review provides an overview of the recent advances in analytical methods and techniques for the detection£¬quantification and location analysis of Ade-to-Ino RNA editing in a wide range of RNA molecules£®The principles£¬advantages£¬limitations and applications of these established methods are discussed£®It is hoped that this review could stimulate the development of analytical methods to decipher Ade-to-Ino RNA editing and expedite the elucidation of the functions of Ade-to-Ino RNA editing in various RNA species£®