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Electrochemical Immunosensor Based on Au-Ag Bimetallic Nanoclusters@CNTs-TiO2 Nanomaterials as Novel Redox Probes |
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DOI: |
KeyWord:bimetallic nanoclusters ?synergistic effect ?Au-Ag NCs@CNTs-TiO2?nanocomposites ?carbohydrate antigen 19-9 |
Author | Institution |
HUANG Xiao-mei,DENG Xiang,DENG Zi-he |
1. Department of Chemistry and Chemical Engineering,Sichuan University of Arts and Science,Dazhou ,China;
2. Key Laboratory of Exploitation and Study of Distinctive Plants in Education Department of Sichuan Province,Dazhou ,China;
3. Key Laboratory of Green Chemistry of Sichuan Institutes of Higher Education,Zigong ,China;
4. College of Chemistry and Chemical Engineering,Shihezi University,Shihezi ,China |
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Abstract: |
A highly sensitive "sandwich" electrochemical immunosensor was constructed for the detection of carbohydrate antigen 19-9(CA19-9),by synthesizing a unique "pearl necklace" structure of gold-silver bimetallic nanoclusters multi-walled carbon nanotubes-titanium dioxide nanocomposites(Au-Ag NCs@CNTs-TiO2 NPs) used as a signal label,carrier and signal amplifier.Firstly,the magnetic Au-Fe3O4?NPs with peroxidase mimetic properties were used to modify the electrode,then the anti-CA19-9 antibody and BSA were immobilized on the electrode surface through chemical bonding,and subsequently,the antibody was bound by specific reaction.Finally,Au-Ag NCs@CNTs-TiO2 NPs/Ab2/BSA biological coupling with redox activity and amplification was combined to form a sandwich immune structure.The experimental results showed that the response signal was effectively amplified by using double peroxide analog enzyme to catalyze H2O2 in the substrate. Under the optimal experimental conditions,CA19-9 showed a good linearity in the concentration range of 0.01-200 U/mL,with a detection limit of 0.003 U/mL.The recoveries of the immunosensor ranged from 94.6% to 105%,with relative standard deviations(RSD) of 4.3%-6.5%,indicating that the immunosensor could be used for the detection of CA19-9 in clinical serum samples. |
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