Analysis of Diarylheptanoids in Zingiberaceae by UPLC£­ ESI£­Q£­TOF MS Combined with Fragmentation Rule

KeyWord:diarylheptanoids  fragmentation rule  UPLC£­ESI£­Q£­TOF MS  zingiberaceae

Abstract:

      According to the mass spectrum data of the standard substance£¬the fragmentation rule of diarylheptanoid was discussed£¬and the component in zingiberaceae plants was analyzed by the fragmentation rule£®The standard samples were injected by syringe pump£®The mass spectrum data of 11 standards were obtained by high resolution and high accuracy electrospray ionization quadrupole time-of-flight tandem mass spectrometry£¨ESI£­Q£­TOF MS£© in positive ion mode£¨ESI+£©£®On the basis of the obtained standards mass spectrum data£¬the cracking behaviors of the standards were analyzed£®According to the cracking behaviors of standard samples£¬four kinds of cracking pathways of diarylheptanoid were summarized£¬and the fragmentation rule of diarylheptanoid was summarized£®Based on the fragmentation rule of diarylheptanoid standards£¬an ultra-high performance liquid chromatography£­electrospray ionization quadrupole time-of-flight mass spectrometry£¨UPLC£­ESI£­Q£­TOF MS£© was established for the rapid identification of diarylheptanoid components in ginger peel£¬Amomum tsao£­ko and Alpinia oxyphylla Miq. 14£¬ 11 and 10 of this kind of components were successfully identified from them£¬respectively£®The obtained results verified the feasibility of this method£®The method is accurate£¬reliable and efficient£¬and it could be used for rapid identification and analysis of diarylheptanoids in zingiberaceae plants£®