Preparation of a Novel Peroxide-like Nanozyme NC@MIL£­100£¨Fe£© and Its Application in Biothiols Analysis

KeyWord:nanozyme  needle coke  MIL£­100£¨Fe£©  glutathione  cysteine

Abstract:

      Enzyme-catalyzed reactions are characteristic of high selectivity and high efficiency£®Specially designed metal£­organic frameworks£¨MOFs£© have catalytic activities similar to natural enzymes£¬and their porous structures also provide a large number of active sites for catalytic reactions£®In this paper£¬a novel nanozyme NC@MIL£­100£¨Fe£© was prepared based on the electrostatic interaction between needle coke£¨NC£© and MOF material£¨MIL£­100£¨Fe£©£©£¬which was applied to the analytical methods development of biothiols£®The inherent enzyme-like activity of NC was made full use for the new material£¬whose lamellar structure could effectively avoid the agglomeration phenomenon of MIL£­100£¨Fe£©£¬improving the overall catalytic effect of the material£®Compared with needle coke and MIL£­100£¨Fe£©£¬NC@MIL£­100£¨Fe£© as nanozyme has a stronger affinity for the substrate and a faster reaction rate£¬thus showing a better peroxidase-like catalytic activity£®Its performance was evaluated with 3£¬3¡ä£¬5£¬5¡ä-tetramethylbenzidine£¨TMB£© and H2O2 as substrates£¬and the Michaelis constants£¨Km£© obtained were 0.27 mmol/L and 0.43 mmol/L£¬respectively£¬which were 1.61 times and 8.6 times smaller than that of horseradish peroxidase£®The material was applied to the development of analytical methods for glutathione and cysteine£¬with the linear ranges of 3£­70 ¦Ìmol/L and 1£­80 ¦Ìmol/L£¬and the detection limits of 0.33 ¦Ìmol/L and 0.22 ¦Ìmol/L£¬respectively£¬showing the advantages of wide linear range£¬low detection limit and good selectivity£®Furthermore£¬this material was successfully applied to the detection of biothiols concentration in human serum with satisfactory results£®