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| Determination of Brilliant Blue FCF and Erythrosine B in Beverage and Candy Samples Using In-situ Effervescence Assisted Microextraction Method Based on Acidic Ionic Liquid |
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| DOI： |
| KeyWord:ionic liquid effervescence assisted microextraction synthetic pigments beverage candy |
| Author | Institution |
| WANG Qing，QIU Bin，HE Yan，WANG Lin-juan，ZHANG Hui，YANG Er-ling，ZHANG Xiao-yuan |
1. The College of Pharmacy，Xiangnan University，Chenzhou ，China; 2. Chenzhou City Center for Disease Control and Prevention，Chenzhou ，China; 3. Research Institute of Shaoguan Huagong High－Tech Industry，Shaoguan ，China; 4. Industrial Technology Research Institute， South China University of Technology，Guangzhou ，China |
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| Abstract: |
| The anion of acidic ionic liquid 1-butyl-3-methylimidazole hydrogen sulfate（［C4MIM］［HSO4］） reacted with carbonate to produce carbon dioxide．The extraction process was promoted by carbon dioxide bubbles．Meanwhile，the cation of ［C4MIM］［HSO4］ reacted in situ with ammonium hexafluorophosphate（NH4PF6） to form hydrophobic ionic liquid 1-butyl-3-methylimidazole hexafluorophosphate ［C4MIM］［PF6］．［C4MIM］［PF6］ was separated from water due to their immiscibility with the water．The extraction phase was collected by centrifugation．An in-situ effervescent assisted microextraction method based on acidic ionic liquid was established for the analysis of brilliant blue FCF and erythrosine B in beverage and candy samples．Effects of the amount of acidic ionic liquid，sodium bicarbonate and salt on the extraction efficiency of the two pigments were investigated．The optimum conditions were as follows：the amount of acidic ionic liquid was 400 mg，the amount of sodium bicarbonate was 0.4 g and the amount of salt was 1.5 g．Under the optimal conditions，there were good linear relationships for brilliant blue FCF and erythrosine B in the range of 0.005－5 μg/mL，with their correlation coefficients not less than 0.993 3．The limits of detection（LODs） and limits of quantitation（LOQs） were in the range of 0.002－0.005 μg/mL and 0.007－0.017 μg/mL，respectively．Additionally，the recoveries for two synthetic pigments at two spiked levels of 0.05 and 0.5 μg/mL ranged from 92.2% to 107%，with the intra-day and inter-day relative standard deviations（RSDs） both 2.2%－8.7%．The proposed method was successfully applied to the detection of two target pigments in beverage and candy samples．The results showed that the method was environmentally friendly，simple and accurate．It could be used for the determination of brilliant blue FCF and erythrosine B in beverage and candy samples． |
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