Study on Timing Difference of Goat Milk MFGM Protein in Lactation Period Based on Ultrahigh Performance Liquid Chromatography£­Quadrupole£­Orbitrap Mass Spectrometry

KeyWord:goat milk  milk fat globule membrane£¨MFGM£© proteins  ultrahigh performance liquid chromatography£­quadrupole£­orbitrap mass spectrometry  proteomics

Abstract:

      High throughput mass spectrometry combined with label-free quantitative technique was used to identify the milk fat globule membrane£¨MFGM£© proteins of goat milk at different lactation stages£®331 and 252 MFGM proteins were quantified in goat milk colostrum and mature milk£¬respectively£¬of which 164 proteins were co-expressed£®The identified MFGM proteins were analyzed by univariate and multivariate analysis£¬and the partial least squares discriminant analysis model was established. Taking 2£¬0.05 and 1.2 as the thresholds of fold change£¬significant difference and variable projection importance analysis value£¬the differential markers in colostrum and mature milk were screened£®Bioinformatics analysis showed that the identified protein markers mainly involved in inflammatory process£¬integrin signaling pathway and galactose metabolism£®The results were of great significance to facilitate people¡¯s in-depth understanding of goat milk protein£¬upgrading the nutritional improvement of milk and its products and the production of breast milk substitutes.