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| Qualitative Non-targeted Screening of 79 Drug Residues in Pork by Ultra-performance Liquid Chromatography Quadrupole Time-of-flight Mass Spectrometry |
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| DOI： |
| KeyWord:ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry （UPLC－Q－TOF MS） non-targeted screening high throughput drug residues pork |
| Author | Institution |
| LIU Jia，WANG Jian-feng，GAO Li-juan，WANG Qiu-shui，FENG Yue-chao，LIU Yan，DING Qi，WANG Ying，SHAO Peng |
Institute of Analysis and Testing，Beijing Academy of Science and Technology，Beijing Center for Physical and Chemical Analysis，Beijing ，China |
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| Abstract: |
| An analytical method was developed for qualitative non-targeted screening of 79 drug residues in pork by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry（UPLC－Q－TOF MS）．The samples were firstly extracted with 0.5%（by volume） formic acid－acetonitrile，then extracted with methanol．After centrifugation，the supernatant was cleaned up with FAVEX－NM50 rapid column of veterinary drug residues，then separated on an Acquity UPLC BEH HSS－C18 column（2.1 mm × 150 mm，1.7 μm），ionized in ESI+ mode，and detected in MSE mode．The calibration curves for 79 drugs had good linearity in the linear range with correlation coefficients（r2） not less than 0.99. The limits of detection and the limits of quantitation were in the ranges of 0.05－10 μg/kg and 0.10－20 μg/kg，respectively．The simulated positive samples and commercial pork samples were screened using the self-built mass spectrometry database．Meanwhile，the commercial pork samples were detected by high sensitivity Xevo TQ－S tandem quadrupole mass spectrometry in multiple reaction monitoring（MRM） mode．Method validation showed that the established method was efficient，rapid and high throughput，which is suitable for the screening and identification of drug residues in pork. |
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