Simultaneous Determination of 4 Antibiotics in Children¡¯s Cosmetics by Ultra-performance Liquid Chromatography£­Tandem Mass Spectrometry with Solid Phase Extraction

KeyWord:antibiotic  children¡¯s cosmetics  ultra-performance liquid chromatography£­tandem mass spectrometry£¨UPLC£­MS/MS£©  purification

Abstract:

      An ultra-performance liquid chromatography£­tandem mass spectrometry£¨UPLC£­MS/MS£© was established for the determination of 4 antibiotics in children¡¯s cosmetics£®The cosmetic samples were extracted with 70% acetonitrile£­water solution£¬and then purified with a SCX column£®The target analytes were separated on a Waters ACQUITY BEH C18£¨2.1 mm ¡Á 100 mm£¬2.5 ¦Ìm£© column using 0.1% formic acid solution and acetonitrile as mobile phases£®The analysis was performed under multiple reaction monitoring£¨MRM£© mode£®Results showed that there were good linear relationships for 4 antibiotics in the respective concentration ranges with their correlation coefficients£¨r£© of 0.999£®The limits of detection£¨LODs£© and limits of quantitation£¨LOQs£© of the method were in the range of 0.005£­0.250 ¦Ìg/g and 0.010£­0.500 ¦Ìg/g£¬respectively. Average recoveries for the 4 antibiotics in 3 kinds of samples at 3 spiked levels ranged from 89.3% to 113%£¬with relative standard deviations£¨RSDs£¬n = 6£© of 0.90%£­7.2%£®The method is effective£¬accurate and specific£¬and it could be used for the determination of 4 antibiotics in children¡¯s cosmetics.