Simultaneous Determination of 37 Kinds of Prohibited Veterinary Drug Residues in Aquacultural ¡°Non-pharmaceutical¡± Inputs by Ultra Performance Liquid Chromatography£­Tandem Mass Spectrometry with Solid Phase Extraction

KeyWord:solid phase extraction  ultra performance liquid chromatography£­tandem mass spectrometry £¨UPLC£­MS/MS£©  ¡°non-pharmaceutical¡± inputs  veterinary drug residue

Abstract:

      An ultra performance liquid chromatography£­tandem mass spectrometry£¨UPLC£­MS/MS£© with solid phase extraction was established for the simultaneous determination of 37 kinds of prohibited veterinary drug residues£¬i.e£®sulfonamides£¬aminools£¬nitroimidazoles£¬benzimidazoles and antiviral classes in aquacultural ¡°non-pharmaceutical¡± inputs£®The samples were extracted with 80% acetonitrile aqueous solution containing 0.2% formic acid and chelated with Mcllvaine£­Na2EDTA buffer£¬then ultrasonically extracted£®The residues contents were separated on a C18 chromatographic column£¬then determined by UPLC£­MS/MS with electrospray ion source in positive and negative ion mode under multiple reaction monitoring mode£®The quantitative information was obtained by matrix-added calibration with external standard£®The correlation coefficients of calibration curves for 37 banned veterinary drugs were not less than 0.991£®The limits of detection and quantitation were in the range of 0.156£­1.94 ¦Ìg/kg and 0.520£­6.47 ¦Ìg/kg£¬respectively£®The spiked recoveries of the method ranged from 71.6% to 119%£¬with the relative standard deviations£¨RSDs£© of 1.2%£­15%£®The method is rapid£¬accurate and sensitive£¬and it could be used for the rapid and accurate screening and detection of 37 kinds of prohibited veterinary drug residues in aquacultural ¡°non-pharmaceutical¡± inputs.