|
| Determination of 11 Anabolic Androgenic Steroids in Compound Feeds by High Performance Liquid Chromatography－Tandem Mass Spectrometry |
| |
| View Full Text Download reader |
| DOI： |
| KeyWord:anabolic androgenic steroids high performance liquid chromatography－tandem mass spectrometry compound feed internal standard method |
| Author | Institution |
| WANG Bo，ZHANG Jing，GONG Song-song，ZHANG Hao-ran，XU Ting，CHEN Si-si，WU Jian-ping，YAN Feng |
Shanghai Animal Disease Prevention and Control Center，Shanghai ，China |
|
| Hits: 542 |
| Download times: 710 |
| Abstract: |
| A high performance liquid chromatography－tandem mass spectrometry（HPLC－MS/MS） was established for the rapid analysis of 11 anabolic androgenic steroids，i.e．testosterone，17-methyltestosterone，boldenone，metandienone，androstenedione，dehydroepiandrosterone，nandrolone，nandrolone 17-propionate，stanozolol，melengestrol，progesterone in compound feeds．The samples were extracted with acetonitrile，then purified with PSA，and finally determined on the machine．The separation of target compounds was performed on an Phenomenex C18（100 mm × 2.1 mm，2.6 μm） by gradient elution，using 0.01% formic acid－acetonitrile system as mobile phase．The 11 anabolic androgenic steroids were detected in positive ESI mode under multiple reaction monitoring（MRM） mode，and quantified by the isotope internal standard method．The results showed that the linear ranges for 11 anabolic androgenic steroids were 1－100 ng/mL，and the correlation coefficients were all 0.999．The limits of detection and the limits of quantitation were 20 μg/kg and 50 μg/kg，respectively．The recoveries for 11 anabolic androgenic steroids in chicken compound feed ranged from 94.5% to 111%，with intra-day relative standard deviations（intra-RSDs） and inter-day RSDs（inter-RSDs） both not more than 13%，while the recoveries for the analytes in pig compound feed ranged from 90.1% to 109%，with intra-RSDs and inter-day RSDs not more than 9.0% and 8.8%，respectively．Testosterone and boldenone were detected in actual samples with the contents of 9.09－14.68 mg/kg and 1.22－1.84 mg/kg，respectively．This method could realize the comprehensive，simple and accurate detection of anabolic androgenic steroids in compound feeds，and provide technical support for the regulation of anabolic androgenic steroids abuse in compound feeds in the future. |
| Close |