Detection of NF£­¦ÊB p50 Using a Biosensing Platform Based on In Situ Nanozyme on PbTiO3 Surface

KeyWord:nanozyme  biosensing  PbTiO3  NF£­¦ÊB p50

Abstract:

      A biosensing platform for the detection of NF£­¦ÊB p50 was constructed£¬based on an in situ nanozyme with adjustable catalytic activity£®The in situ nanozyme was obtained by combining K4Fe£¨CN£©6 onto the surface of PbTiO3£®Generally£¬the attachment of deoxyribonucleoside 5'-monophosphate£¨dNMP£© onto the PbTiO3 surface may prevent the spontaneous formation of nanozyme on its surface£¬while in the presence of NF£­¦ÊB p50£¬dNMP is hardly to be generated£¬which allows K4Fe£¨CN£©6 combining smoothly to the surface of PbTiO3£¬and a spontaneously formed nanozyme on PbTiO3 surface catalyzes TMB oxidation for the quantitative detection of NF£­¦ÊB p50£®The results showed that the detection linear range of this method for NF£­¦ÊB p50 was 3.0 pmol/L£­10 nmol/L£¬while the limits of detection£¨S/N = 3£© was 1.2 pmol/L£®The spiked recoveries for real samples ranged from 99.1% to 102%£¬with relative standard deviations not more than 5.3%£®This method provides a new strategy in label-free£¬immobilization-free mode for the detection of NF£­¦ÊB p50 with amplified signal£¬which not only has high sensitivity£¬good selectivity and simple operation£¬but also has an application potential in the detection of actual samples.