Preparation of a Covalent Organic Framework TpBD-£¨NH2£©2-D-£¨£­£©-¦Á-phenylglycine and Its Application Used as Chiral Stationary Phase for High Performance Liquid Chromatography

KeyWord:covalent organic framework materials  chiral stationary phase  high performance liquid chromatography  chiral separation

Abstract:

      In this work£¬a covalent organic framework£¨COF£© material TpBD-£¨NO2£©2 was firstly synthesized by solvothermal method£¬which was then reduced to TpBD-£¨NH2£©2£¬and finally£¬a chiral COF material TpBD-£¨NH2£©2-D-£¨£­£©-¦Á-phenylglycine was obtained by chirally modifying the TpBD-£¨NH2£©2 with D-£¨£­£©-¦Á-phenylglycine£®Three COF materials£¬i.e£®TpBD-£¨NO2£©2£¬TpBD-£¨NH2£©2 and TpBD-£¨NH2£©2-D-£¨£­£©-¦Á-phenylglycine were characterized£¬and a chiral stationary phase of TpBD-£¨NH2£©2-D-£¨£­£©-¦Á-phenylglycine was prepared by ¡°net-wrap method¡±£®In order to investigate the separation performance of the stationary phase£¬it was used to prepare a chiral column for high performance liquid chromatography£¨HPLC£©£¬n-hexane£­isopropanol£¨9¡Ã1£¬by volume£© and methanol£­water£¨9¡Ã1£¬by volume£© were used as mobile phases£®13 chiral compounds and 5 benzene positional isomers were separated on this column with good reproducibility£®The experiment showed that the separation effect of TpBD-£¨NH2£©2-D-£¨£­£©-¦Á-phenylglycine chromatographic column was excellent. It was of a certain research significance to use the chiral COF material TpBD-£¨NH2£©2-D-£¨£­£©-¦Á-phenylglycine as HPLC stationary phase for the separation of chiral substances and benzene isomers.