Rapid Screening of Prostaglandin F2¦Á Compounds in Eyelash Cosmetics by Ultra-performance Liquid Chromatography£­ Quadrupole Time-of-flight Mass Spectrometry

KeyWord:prostaglandin F2¦Á  cosmetics  ultra-performance liquid chromatography£­quadrupole time-of-flight mass spectrometry

Abstract:

      A high-throughput method for rapid and qualitative screening of nine prostaglandin F2¦Á compounds£¬i.e. bimatoprost£¬travoprost£¬tafluprost£¬tafluprost ethyl amide£¬latanoprost£¬bimatoprost acid methyl ester£¬cloprostenol isopropyl ester£¬15-keto travoprost£¬N-norbimatoprost in eyelash cosmetics was established by ultra-performance liquid chromatography£­quadrupole time-of-flight mass spectrometry£¨UPLC£­Q£­TOF MS/MS£©£®The samples were ultrasonically extracted with acetonitrile£¬separated on a Waters ACQUITY UPLC BEH C18£¨2.1 mm ¡Á 100 mm£¬1.7 ¦Ìm£© chromatographic column with 2 mmol/L ammonium formate aqueous solution containing 0.05% formic acid£­acetonitrile as the mobile phase£¬then data-acquisitioned by quadrupole time-of-flight mass spectrometry in MSE mode using electrospray ionization source in positive ion mode£¨ESI+£©£¬and a high-throughput screening database for 9 prostaglandin F2¦Á compounds was established£®There were good linear relationships for the 9 analytes in their respective concentration ranges£¬with correlation coefficients£¨r2£© all greater than 0.99£®The detection limits and the quantitation limts of 9 analytes were in the ranges of 0.075£­0.75 ¦Ìg/g and 0.25£­2.5 ¦Ìg/g£¬respectively£®The screening database collected and established by high-resolution mass spectrometry can screen 9 kinds of prostaglandin F2¦Á compounds in cosmetics without reference substances£®The results showed that the established method has good selectivity in high-throughput qualitative screening of 48 batches of eyelash cosmetics with good specificity£®The method is suitable for the screening and analysis of prostaglandin F2¦Á compounds in cosmetics£¬and it could provide a technical support for the qualitative analysis of five prostaglandin F2¦Á compounds in cosmetics.