Preparation of Matrix Reference Material for Nicarbazin Residues in Chicken Muscle

KeyWord:chicken  nicarbazin  matrix reference material  liquid chromatography£­tandem mass spectrometry£¨LC£­MS/MS£©

Abstract:

      A development method for nicarbazin residue matrix reference materials in chicken muscle was established in this paper to fill the gap of those in China£®Positive animals with nicarbazin residues were obtained by feeding white feather broiler chickens with nicarbazin drug powder in feed and allowing the drug to metabolize naturally in the chickens£®A batch of 355 chicken lyophilized powders containing nicarbazin residues were prepared by taking chicken breast meat and making minced meat£¬through the processes of homogenization£¬freeze-drying£¬crushing£¬sieving£¬packaging and irradiation sterilization£®Homogeneity assessments£¬stability monitoring£¬characterization and uncertainty analysis were carried out according to the requirements of national reference materials£®The contents of nicarbazin in the samples were determined by liquid chromatography£­tandem mass spectrometry£¨LC£­MS£©£¬which were cooperatively certified by 8 expert laboratories£®The results showed that the homogeneity and stability of this batch of reference materials were good and they could be kept stable for 14 days under the temperature conditions of -70 ¡æ£¬4 ¡æ£¬25 ¡æ and 50 ¡æ£¬and up to 12 months when stored at -18 ¡æ£¬which was in accordance with the relevant requirements of the national reference materials£®The certified value of nicarbazin in the reference materials was finally determined to be £¨157.7 ¡À 11.0£© ¦Ìg¡¤kg-1£¨k = 2£©£®The reference material could be used for optimization of detection methods for nicarbazin£¬and capacity evaluation on nicarbazin residue testing laboratories£¬which is of great significance to the detection of nicarbazin residues in food.