Determination of 21 Anesthetics in Skin Care Products by QuEChERS/High Performance Liquid Chromatography£­Tandem Mass Spectrometry

KeyWord:QuEChERS  high performance liquid chromatography£­tandem mass spectrometry£¨HPLC£­MS/MS£©  anesthetic  skin care product

Abstract:

      A QuEChERS/high performance liquid chromatography£­tandem mass spectrometry£¨HPLC£­MS/MS£© was established for the rapid and simultaneous determination of 21 anesthetics in skin care products£®The samples were dispersed with water£¬then extracted with acetonitrile solution containing 1.0%£¨by volume£© formic acid by ultrasound extraction£¬and purified with anhydrous magnesium sulfate£¬octadecyl silane£¨C18£© and primary secondary amine£¨PSA£©£¬and finally seperated on an Acclaim Mixed£­Mode WCX£­1 column£¨150 mm ¡Á 2.1 mm£¬3 ¦Ìm£© which could offer multiple retention mechanisms including reversed-phase£¨RP£©£¬weak cation-exchange£¨WCX£© and HILIC£®The mobile phases were 20 mmol¡¤L-1 ammonium formate£¨adjusting to pH 4.0£© and acetonitrile£¨cantaining 0.035% formic acid£©£®The samples were detected in electrospray positive ion mode under multiple reaction monitoring£¨MRM£© mode£¬and quantified by matrix-matched standard solutions and external standard method£®There were good linear relationships for 21 anesthetics in the concentration range of 0.5£­100 ng¡¤mL-1£¬with their correlation coefficients£¨r£© larger than 0.99£®The detection limits and quantitation limits were 0.1£­3 ¦Ìg¡¤kg-1 and 0.3£­10 ¦Ìg¡¤kg-1£¬respectively£®The average recoveries at three spiked levels for the 21 analytes in three matrices ranged from 84.3% to 109%£¬with relative standard deviations of 0.70%£­7.1%£¨n = 6£©£®The method is simple to operate£¬and it is suitable for the daily detection of 21 anesthetics in skin care products.