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| Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection |
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| DOI： |
| KeyWord:Chonglou Xiaoliu Formula saponins ultra performance liquid chromatography evaporative light scattering detection |
| Author | Institution |
| XU Ai-li，CAO Ying-nan，ZHANG Su-zhong，QIU Jin-yan，XIE Can-hui，BI Xiao-li，XIAO Guan-lin |
1. Guangdong Province Engineering Technology Research Institute of T.C.M，Guangzhou ，China; 2. College of Pharmacy，Guangzhou Xinhua University，Guangzhou ，China; 3. Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine，Guangzhou ，China; 4. The Fifth Clinical Medicine School，Guangzhou University of Chinese Medicine，Guangzhou ，China |
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| Abstract: |
| An ultra performance liquid chromatography（UPLC） coupled with evaporative light scattering detection（ELSD） was established for the simultaneous determination of 5 saponins，i.e．polyphyllin I，polyphyllin Ⅱ，polyphyllin Ⅶ，saikosaponin a and saikosaponin d in Chonglou Xiaoliu Formula. The samples were extracted with methanol， then separated on a Waters Cortecs UPLC C18（150 mm × 2.1 mm，1.6 μm） column with acetonitrile－water as the mobile phase by gradient elution，finally determined by ELSD and quantified by external standard calibration method．The results showed that there were good linear relationships for 5 saponins in the ranges of 7.117 4－177.94 μg/mL，9.407 2－235.18 μg/mL，8.872 6－221.82 μg/mL，7.486 2－187.15 μg/mL and 9.761 3－244.03 μg/mL，respectively，with their correlation coefficients（r） of 0.999 2－0.999 8．The limits of detection and the limits of quantitation were in the ranges of 0.7－1.0 μg/mL and 1.8－2.4 μg/mL，respectively．The average recoveries were 93.9%，103%，102%，104% and 105%，with their relative standard deviations（RSDs） of 3.8%，4.0%，4.2%，3.6% and 3.2%，respectively．With the advantages of simplicity，rapidness and accuracy，the method could be applied in simultaneous analysis of 5 saponins in Chonglou Xiaoliu Formula and effective elimination of the impurity interference．And it also provides a reference for the quality evaluation on Chonglou Xiaoliu Formula． |
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