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Simultaneous Determination of 9 Self-heating Compounds Including Capsaicin in Cosmetics by High Performance Liquid Chromatography |
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KeyWord:self-heating compounds cosmetics high performance liquid chromatography(HPLC) |
Author | Institution |
WU Zhi-shan,WU Jiao-jiao,YIN Wei-cheng,YIN Shuai,LI Zhuo-fang,JIANG Cheng-jun |
1. Hunan Institute for Drug Control,Changsha ,China; 2. Food and Drug Inspection in Yueyang,Yueyang ,China |
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Abstract: |
A high performance liquid chromatographic(HPLC) method was established for the determination of 9 self-heating compounds,including capsaicin in cosmetics.The samples were ultrasonically extracted with 80% methanol,and then filtered.The target compounds were separated on a SymmetryShieldTMRP18(250 mm × 4.6 mm,5 μm) with a mobile phase consisting of acetonitrile and 0.5% formic acid by gradient elution,and analyzed by HPLC with a PDA detector.Under the optimal conditions,good linear relationships were obtained for nine compounds in the concentration range of 0.2-100 μg/mL,with correlation coefficients(r2) not less than 0.999 5.The limits of detection(LODs) were in the range of 0.001 0-0.003 4 mg/kg.The spiked recoveries for water,lotion and cream matrixes at three levels of 0.004,0.02,1 mg/kg ranged from 75.6% to 115%,with relative standard deviations(RSDs,n = 3) of 0.083%-7.0%.This method is accurate and sensitive,and is suitable for the qualitative and quantitative analysis of 9 self-heating compounds in cosmetics. |
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